This book provides a holistic overview of current state of the art and practice in malware research as well as the challenges of malware research from multiple angles. It also provides step-by-step guides in various practical problems, such as unpacking real-world malware and dissecting it to collect and perform a forensic analysis. Similarly, it includes a guide on how to apply state-of-the-art Machine Learning methods to classify malware. Acknowledging that the latter is a serious trend in malware, one part of the book is devoted to providing the reader with the state-of-the-art in Machine Learning methods in malware classification, highlighting the different approaches that are used for, e.g., mobile malware samples and introducing the reader to the challenges that are faced when shifting from a lab to production environment.